Analysis of Garlic Clove Homogenates
Table 5. Analysis of variance
Whilst the differences in primary flavour compounds
between isozyme groups appear limited to the effect
of the initial level of the 1-propenyl cysteine
sulphoxide in intact garlic tissue, the relationships
between common morphological characteristics and
between morphological characteristics and alkyl
groups (derived from alkyl cysteine sulphoxides)
appear more evident. Tables 6 - 10 show the calculated
correlation coefficients ( r ) for all available
features of the samples (Table
4) and whilst a number of significant correlations
can be seen, relationships of this nature in plants
are seldom linear and so the figures should be viewed
with caution until further studies have been undertaken.
It is worth noting at this point that Saghir
has reported on the use of chemical and anatomical
data as valuable indicators of taxonomic relationships
in alliums and has suggested a correlation between
the proportions of alkyl sulphides found in tissue
samples and morphological features such as inflorescence
and bulb structure and leaf anatomy.
From a practical
standpoint there were some elements of the method
that require close attention and possibly some
for HPLC injection must be totally dry as water
contamination causes severe ‘spiking’
of the traces,
filter must be cleaned in 20% nitric acid in
an ultrasonic bath for 30 minutes every day.
Such is the level of deposit that failure to
clean (or replace) this filter regularly causes
increasing back pressure and drifting retention
of the solvents employed, only glass syringes
should be used for HPLC injection and,
every effort was made to ensure consistent final
sample volume this method can inherently lead
to error at this stage.
The tests have shown that there
is a significant difference between the levels
of some primary flavour compounds found in garlic
tissue homogenates and some principal isozyme
There was an insufficient number of representative
samples from secondary isozyme groups to enable
statistical analysis beyond the primary groups.
A representative from primary isozyme group 2
would aid future investigations.
Whilst every effort was made to confirm the identities
of the clones used, future studies should include
isozyme testing as an accurate means of grouping.